Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | SPR assay |
| Description: | SPR measurements wereperformed on BIAcore T100 instrument (BIAcore GE Healthcare), at25 C on series S NTA chips (certified) according to provider's protocolswith 10mMHEPES, pH 7.4, 150mMNaCl, 500 μMEDTA, 0.05%Tween-20, and 1% DMSO as a running buffer. Histidine-tagged Grp78was immobilized on the sensor surface; reference surfaces withoutimmobilized Ni2+ served as controls for nonspecific binding and refractiveindex changes. Concentrations of inhibitors (0--200 μM) weretypically injected over the sensor chip at 35 μL/min. Zero concentrationsamples were used as blanks. The sensor surface was regenerated betweenexperiments by injections of 0.1 mg/mL trypsin and 50% DMSO. Dataprocessing was performed using BIAevaluation 2.1 software (BIAcore GEHealthcare Bio-Sciences Corp.) by globally fitting the entire inhibitorconcentration series data set to the steady state affinity model. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||