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Assay Method Information

Assay Name:  p110alpha (alpha) PI3K Binding Assay
Description:  PI3K Binding assays are intended for determining the biochemical potency of small molecule PI3K inhibitors. The PI3K lipid kinase reaction is performed in the presence of PIP2:3PS lipid substrate (Promega #V1792) and ATP. Following the termination if the kinase reaction, turnover of ATP to ADP by the phosphorylation of the lipid substrate is detected using the Promega ADP-Gloż (Promega #V1792) assay.
Affinity data for this assay
 

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Last update November 1, 2007
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