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| | Heme Oxygenase |
| Description: | Heme oxygenase activity in rat spleen and brain microsomal fractions was determined by the quantitation of CO formed from the degradation of methemalbumin (heme complexed with albumin). Spleen and brain (SpragueâDawley rats) microsomal fractions were prepared according to the procedure outlined by Appletonet al. Protein concentration of microsomal fractions was determined by a modification of the Biuret method. Incubations for HO activity analysis were performed under conditions for which the rate of CO formation [pmol CO â (min mg protein)] was linear with respect to time and microsomal protein concentration. Briefly, reaction mixtures (150 lL) consisting of 100 mM phosphate buffer (pH 7.4), 50 lM methemalbumin, and 1 mg â mL protein were preincubated with the inhibitors at final concentrations ranging from 0.1 to 100 lM for 10 min at 37 degC. Reactions were initiated by adding NADPH at a final concentration of 1 mM, and incubations were performed for an additional 15 min at 37 |
| Affinity data for this assay | |
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