Assay Method Information |
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| Protease Inhibition Assay |
Description: | Protease activity was measured by continuous chromogenic assay. The chromogenic peptide His-Lys-Ala-Arg-Val-Leu- (p-NO2-Phe)-Glu-Ala-Nleu-Ser (Bachem) was used as a substrate. Reactions were followed by the decrease of absorbance at 300 nm measured by spectrophotometer. Experimental results were analyzed by the nonlinear regression analysis, using a mathematical model for tight binding inhibitors. |
Affinity data for this assay | |
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