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Assay Method Information |
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| | Enzyme Activity Assay |
| Description: | The formation of ADP from ATP was quantified using a coupled enzyme assay (DiscoverX) in with a fluorescent resorufin dye is generated from the interaction of ADP with hydrogen peroxide and 10-acetyl-3,7-dihydroxy-phenoxazine (excitation and emission wavelenghts of 540 and 590 nm). |
| Affinity data for this assay | |
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