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Assay Method Information |
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| | Inhbition Assay |
| Description: | PfHGXPRT activity was measured using spectrophotometric assay observing the conversion of xanthine and 5-phospho-alpha-D-ribose-1-pyrophosphate to xanthosine-5'-monophosphate and inorganic pyrophosphate (PPi) at 247nm or the conversion of guanine and PRPP to guanosine-5'-monophosphate and PPi on a Varian Cary 100 spectrophotometer. |
| Affinity data for this assay | |
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