Assay Method Information |
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| Fluorescence Polarization (FP) Assay |
Description: | The labeled fragment can be distinguished from full-length MKK by a change in fluorescence polarization. MKK6 was used as a substrate because it could produce large quantities of the full-length protein, sufficient for HTS. MKK6 inhibitors do not bind in the active site but in a separate exosite. |
Affinity data for this assay | |
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