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Assay Method Information

Assay Name:  PHD Enzymatic Assay
Description:  PHD1,2,3 activity was measured utilizing homogeneous time-resolved fluroescence energy transfer techology by detecting the trans-4-hydroxylatio of HIF-1alpha residue pro564 in biotin-hHIR-1alpha(558-574)(Biotin-DLEMLAPYIPMDDDFQL)peptide substrate resulting in a recognition by the Europium-tagged Von Hippel Lindau, Elongin B and Dlongin C heterotrimeric complex (VCB-Eu complex)
Affinity data for this assay
 

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Last update November 1, 2007
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