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| | IC50 Assay |
| Description: | An appropriate amount of enzyme (4uM) was pre-incubated with the (5R)- or 5(S)-penicilloic acid in the assay buffer (50mM HEPES-NaOH buffer (pH 7.2) supplemented with 0.01% Triton-100) at room temperature for 10 min. Nitrocefin (100uM) was added to initiate the assay. The IC50 values were determined using the software package Graph Prism 5.01. |
| Affinity data for this assay | |
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