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| | IC50 Assay |
| Description: | All reactions were carried in a volume of 2 mL stirred at 23°C with approximately 2.040 Units of 12-hLOX, 4.200 Units of 15-hLOX-1, and 6.600 Units of 15-hLOX2. Reactions with 12-hLOX were carried out in 25mM HEPES (pH 8.0), 0.01% Triton X-100, and 10 µM arachidonic acid. Reactions with 15-hLOX-1 and 15-hLOX-2 were carried out in 25 mM HEPES (pH 7.5), 0.01% Triton X-100, and 10 µM arachidonic acid and 30 µM arachidonic acid, respectively. IC50 values were determined by the enzymatic rate at various inhibitor concentrations and plotted against inhibitor concentration, followed by a hyperbolic saturation curve. |
| Affinity data for this assay | |
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