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Assay Method Information

Assay Name:  DXP Synthase Inhibition Assay
Description:  Reaction mixtures containing HEPES (100mM, pH 8.0, 2mM MgCl2, 5mM NaCl), ThDP (1mM), BSA (1mg/mL), DMSO (10%, v/v), ᴅ-GAP (30µM), pyruvate (80µM), and various concentrations of nitroso inhibitor were preincubated at 37°C for 5 min. Enzyme reactions were initiated by addition of DXP synthase (0.1µM). Aliquots (150µL) of the enzymatic mixture were removed between 0.5 and 3 min and quenched in ice-cold methanol (150µL).
Affinity data for this assay
 

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Last update November 1, 2007
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