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Assay Method Information

Assay Name:  Kinase Assays
Description:  ATP (6.021 μM) and substrate (457.7 nM) were used. Kinase, substrate peptide, and inhibitor were added in a 384-well plate. The reaction was started by the addition of ATP and incubated for 30 min, followed by the addition of an antiphosphotyrosine antibody labeled with europioum cryptate and streptavidin labeled with fluorophore XL665. The fluorescence of the samples was measured at 620 nm (Eu-labeled antibody) and 665 nm (XL665-labeled streptavidin).
Affinity data for this assay
 

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Last update November 1, 2007
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