Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | α-Gal A Assay |
| Description: | For inhibition assay, 0.1% Triton X-100 extracts were mixed with 4-MU substrates ( 5 mM 4-MU α-ᴅ-galactopyranoside and 0.1 M N-acetyl-ᴅ-galactosamine in 0.1 M citrate buffer (pH 4.5)) in absence or presence of increasing concentrations of DGJ derivatives. For heat-induced degradation, extracts were incubated in 0.1 M citrate buffer (pH 7) at 48 °C for the time indicated. The incubation was terminated by adding 0.1 M citrate buffer (pH 4.5). The absorbance was measured at 340 nm and 460 nm. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||