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Assay Method Information

Assay Name:  CB1 & 2 Binding Assays
Description:  In this assay, 18 ug of crude membrane expressing hCB1 or hCB2 receptors were re-suspended in 300 uL of binding buffer containing 50 mM Tris-HCl, 2.5 mM TA, 5 mM MgCl2, 0.5 mg/mL fatty acid free BSA (pH 7.4) and co-incubated with with test compounds at 3 uM, hit compounds at 0.1 nM - 100 uM concentration or vehicle an0.5 nM of [3H]-CP-55940 (168 Ci/mmol) for 2 h at 30 C.
Affinity data for this assay
 

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Last update November 1, 2007
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