Assay Method Information |
|
| CB1 & 2 Binding Assays |
Description: | In this assay, 18 ug of crude membrane expressing hCB1 or hCB2 receptors were re-suspended in 300 uL of binding buffer containing 50 mM Tris-HCl, 2.5 mM TA, 5 mM MgCl2, 0.5 mg/mL fatty acid free BSA (pH 7.4) and co-incubated with with test compounds at 3 uM, hit compounds at 0.1 nM - 100 uM concentration or vehicle an0.5 nM of [3H]-CP-55940 (168 Ci/mmol) for 2 h at 30 C. |
Affinity data for this assay | |
---|---|
If you find an error in this entry please send us an E-mail |
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |