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Assay Method Information

Assay Name:  Quatitative Inhibition Analysis Assay
Description:  The reaction mixture contained DNA (1.25 uM or as specified), NTP (110 uM or as specified), 50 mM NaCl, 150 mM potassium glutamate, buffer [20 mM CAPS (pH 8.8) or as specified], and divalent metals (2 mM Mn2+ and 1 mM Mg2+). The assay was performed in 96-well plates with 4 uM Ba DnaG.
Affinity data for this assay
 

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Last update November 1, 2007
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