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Assay Method Information |
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| | E.coli MurC assay |
| Description: | The reactions (25 μL) were carried out in 25 mM Tris-HCl pH 8.0, 10 mM ammonium sulfate, 1.25 mM DTT, 0.002% Brij-35, 10 mM MgCl2, 40 μM UNAM, 100 μM ATP, 40 μM L-alanine and 15 nM enzyme in 384-well plate at 25 C for 50 minutes. |
| Affinity data for this assay | |
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