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| | 20S proteasome chymotrypsin-like inhibition assay |
| Description: | The assay was carried out in 50 uL volume, where 1 uL compound was added to 10 uL purified human proteasome (25 ug/mL) and incubated for 15 min and then combined with 39 uL synthesized substrate Suc-Leu-Leu-Val-Tyr-AMC (50 uM). The absorbance was measured 355 nm excitation and 460 nm emission. |
| Affinity data for this assay | |
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