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Assay Method Information |
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| | Kinetic Competitive Displacement Assay |
| Description: | The method was a competitive displacement assay used to determine binding affinities of other inhibitors relative to that of GW0385. The inhibitor of unknown affinity was used to displace [3H]GW0385 from enzyme-bound [3H]GW0385 (E[3H]GW0385). From the concentration of E[3H]GW0385 at equilibrium, the concentrations of enzyme-bound and free inhibitors were calculated, and the ratio of the Ki value of the unknown to that of GW0385 was determined (Ki,unknown/Ki,GW0385). |
| Affinity data for this assay | |
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