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| | Fluorescence Polarization-Based Assays |
| Description: | An in vitro fluorescence polarization (FP) based binding assay was used to test the binding ability of the compounds of the present invention to certain IAP proteins (Nikolovska-Coleska et al., Anal. Biochem. 332:261-73 (2004)). he FP-based assay for XIAP BIR3 protein was described in detail previously (Nikolovska-Coleska et al., Anal. Biochem. 332:261-73 (2004)). Briefly, 5-carboxyfluorescein was coupled to the lysine side chain of a mutated Smac peptide with the sequence (AbuRPFK-Fam) and this fluorescently tagged peptide (named SM5F) was used as the fluorescent tracer in FP-based binding assay to XIAP BIR3. |
| Affinity data for this assay | |
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