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Assay Method Information

Assay Name:  HDAC Enzyme Activity Assay
Description:  For the enzyme assay, 10 L of the enzyme fraction was added to 1 L of fluorescent substrate (2 mM Ac-KGLGK(Ac)-MCA) and 9 L of histone deacetylase buffer, and the mixture was incubated at 37 C for 30 min. The reaction was stopped by the addition of 30 L of trypsin (20 mg/mL) and incubated at 37 C for 15 min. The released amino methyl coumarin (AMC) was measured using a fluorescence plate reader. DTT was added simultaneously with drugs to the enzyme solutions
Affinity data for this assay
 

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Last update November 1, 2007
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