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Assay Method Information

Assay Name:  alpha-Glucosidase Assay
Description:  10 無 of test samples (5 mg/mL DMSO solution) were reconstituted in 100 無 of 100 mM-phosphate buffer (pH6.8) in 96-well microplate and incubated with 50 無 ofcrude intestinal α-glucosidase for 5 min before 50 無 substrate (5 mM, p-nitrophenyl-α-D-glucopyranoside prepared in same buffer) was added. Release of p-nitrophenol was measured at 405 nm spectrophotometrically for 5 min after incubation with substrate. Individual blanks for test samples were prepared to correct background absorbance where substrate was replaced with 50 無 of buffer. Control sample contained 10 無 DMSO in place of test samples
Affinity data for this assay
 

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Last update November 1, 2007
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