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| | Acetylcholinesterase Inhibition Assay |
| Description: | AChI and 5,5'-dithio-bis(2-nitro-benzoic)acid (DTNB) were used as substrate for this enzymatic reaction. To this end, 100 µL of Tris/HCl buffer (1 M, pH 8.0) and 10 µL of novel bromophenols solution at different concentrations and 50 µL AChE (5.32 × 10^-3 U) solution were mixed and incubated for 10 min at 25 °C. Then 50 µL of DTNB (0.5 mM) was transferred. Then the reaction was initiated by the addition of 50 µL of AChI. The hydrolysis of AChI was recorded spectrophotometrically by the formation of yellow 5-thio-2-nitrobenzoate anion as the result of the reaction of DTNB with thiocholine at a wavelength of 412 nm. |
| Affinity data for this assay | |
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