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| | Hemoglobin Assay |
| Description: | The assay mixture contained L-arginine (10 μM), NADPH (100 μM), oxyhemoglobin (0.125 mg/mL), tetrahydrobiopterin (10 μM), and different amounts ofinhibitors. The final volume was adjusted to 600 μL with 100 mM Hepes buffer (pH 7.4). The enzymatic reaction was initiated by addition of 10 μL of an iNOS stock, and the rate of NO production was monitored by the change in absorbance at 401 nm in the initial 60 s on a spectrophotometer at 37 °C. |
| Affinity data for this assay | |
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