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Assay Method Information |
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| | Steady State Kinetics |
| Description: | Steady state kinetics were performed on an Agilent 8453 diode array spectrophotometer in sodium phosphate buffer (50 mM, pH 7.2) and a 1 cm pathlength cuvette. Inhibitor kinetics were performed using nitrocefin (NCF) as a reporter substrate (Δε482 = 17400 M^−1cm^−1). Production formation from NCF hydrolysis at an initial concentration of 100 μM in the presence of 0.05 μg/mL BlaC was assessed at 482 nm over a period of 300 s. |
| Affinity data for this assay | |
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