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Assay Method Information |
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| | Aurora B Kinase Assay |
| Description: | The kinase assay was performed using the EMD Millipore KinaseProfiler service assay protocol. Aurora B kinase was supplied by EMD Millipore Corp. The substrate for phosphorylation, AlaLysArgArgArgLeuSerSerLeuArgAla, was 30 µM and the concentration of ATP was 10 µM. All experiments were repeated three times at four different concentrations (0, 5, 10, and 40 µM). |
| Affinity data for this assay | |
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