Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | Fluorophore Displacement Assay |
| Description: | Briefly, steady-state fluorescence spectra of ANS binding was monitored by measuring the increase in fluorescence signal between 450550 nm following excitation at 400 nm while for DAUDA binding was measured between 450600 nm following excitation at 335 nm. Spectrophotometer slit widths were set to 5 and 10 nm for the excitation and emission monochromators, respectively. DAUDA (0.025 mM) and ANS (0.25 mM) were titrated into a hIFABP solution at a protein concentration of 0.5 u1 μM in buffer (20 mM MES pH 5.5, 50 mM NaCl) at 20 C |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||