Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | Akt1 Inhibitory Activity Assay |
| Description: | In vitro kinase assays were carried out using HTScan PKB/Akt1 Kinase Assay kit (Cell Signaling Technology). Active recombinant Akt1 kinase (GST fusion protein, 4 ng) in 8 µL of 2.5× kinase buffer [62.5 mM Tris-HCl (pH 7.5), 25 mM MgCl2, 12.5 mM β-glycerophosphate, 0.25 mM Na3VO4, 5 mM dithiothreitol (DTT)] was mixed with 2 µL of dimethyl sulfoxide (DMSO) vehicle or each of the compound (indicated concentrations), incubated at room temperature for 5 min, and 10 µL of ATP/substrate cocktail (20 mM ATP, 3 mM eNOS served as substrate) was added.After incubation at room temperature for 30 min, add 20 µL of 50mM ethylenediaminetetraacetic acid (EDTA) (pH 8.0) and terminate the reaction. Then, PKB/Akt1 kinase activity was analyzed according to the manufacturer's instructions. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||