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Assay Method Information |
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| | IC50 Assay |
| Description: | The reactions were done in a volume of 2 mL and constantly stirred using a magnetic stir bar at room temperature (23 °C). Reactions with the crude, ammonium sulfate precipitated 5-LOX were carried out in 25 mM HEPES (pH 7.3), 0.3 mM CaCl2, 0.1 mM EDTA, 0.2 mM ATP, 0.01% Triton-X-100, and 10 lM AA. The concentrations of AA for 5-LOX assays were quantitatively determined by allowing the enzymatic reaction to go to completion. |
| Affinity data for this assay | |
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