new BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  Direct Competition Assay
Description:  The Ki for each inhibitor was determined by direct competition assays under steady-state conditions. The initial velocity was measured in the presence of a constant concentration of enzyme (3 nM for VIM-2 and 4 nM for VIM-24) with increasing concentrations of inhibitor (5−60 μM) against a fixed concentration (5KM) of the indicator substrate, NCF, as previously described. The reactionswere started by addition of VIM-2 or VIM-24 to substrate or to mixtures of substrate and inhibitor.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.