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Assay Method Information |
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| | Inhibition Assays |
| Description: | Reactions were conducted similarly to the kinetic assays but the reaction consisted of 10 無 assay buffer, 5 無 inhibitor solution, 5 無 of 10 nM enzyme, and 30 無 of substrate. The reaction was incubated at 37 蚓 for 6 hours to allow the reaction to reach equilibrium and then quenched with 100 無 of 0.2 M sodium carbonate. |
| Affinity data for this assay | |
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