Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | Membrane Autophosphorylation Assay. |
| Description: | The assay was using Swiss 3T3 cell membranes harboring EGF-R or PDGFR-beta. Receptor autophosphorylation was initiated by addition of [gamma-32P]ATP in the presence of EGF or PDGF. In order to test the effects of tyrphostins, these were added 15 min before addition of the growth factors. For quantification of radioactivity in the reaction, a PhosphorImager was used through electrophoresis gels. IC50 is the inhibitor concentration which inhibits 50% of EGF-R or PDGFR-beta kinase activity. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||