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Assay Method Information |
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| | Modified Ellman Assay |
| Description: | For rat AChE or BuChE inhibition assays, a reaction mixture (100 μL) containing acetylthiocholine iodide (1 mmol/L, 30 μL) (J&K Scientific) or butyrylthiocholine iodide (1 mmol/L, 30 μL) (TCI Shanghai Development), phosphate-buffered solution(0.1 mmol/L, pH = 7.4, 40 lL), 5% homogenate or 25% serum (10 μL), and different concentrations (DMSO < 1%) of test compounds (20 μL) was incubated at 37 °C for15 min. Then, 5,5'-dithiobis-2-nitrobenzoic acid (DTNB, 0.2%, 30 μL) (J&K Scientific) was added. |
| Affinity data for this assay | |
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