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Assay Method Information

Assay Name:  α-Glucosidase Inhibitory Activity
Description:  The 135 ÁL of 50 mM phosphate saline buffer pH (6.8) was added in the 96-well plate and 20 ÁL of test sample with 70% DMSO added into the wells. The solution of Enzyme 20 ÁL was added into the wells, and incubated the plate for 15 min. After incubation pre-read of the plate was taken by the spectra max. Afterward 25 ÁL of the substrate (pNPG) was added and a reading was taken on spectra max at 400 nm for 30 min. The normal reading is taken and the percent inhibition was calculated.
Affinity data for this assay
 

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Last update November 1, 2007
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