new BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  Acetylcholinesterase Assay
Description:  The AChE inhibition activity was determined according to the Ellman's method with slight modifications. Total volume of the reaction mixture was 100 無. It contained 60 無 Na2HPO4 buffer with concentration of 50 mM and pH 7.7. Ten 無 test compound (0.5 mM well-1) was added, followed by the addition of 10 無 (0.005 unit well-1) enzyme. The contents were mixed and preread at 405 nm. Then contents were pre-incubated for 10 min at 37 蚓. The reaction was initiated by the addition of 10 無 of 0.5 mM well-1 substrate (acetylthiocholine iodide), followed by the addition of 10 無 DTNB (0.5 mM well-1). After 30 min of incubation at 37 蚓, absorbance was measured at 405 nm. Synergy HT (BioTek, USA) 96-well plate reader was used in all experiments. All experiments were carried out with their respective controls in triplicate.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.