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Assay Method Information

Assay Name:  alpha-Glucosidase Inhibition Assay
Description:  The alpha-glucosidase inhibition assay had been carried out using baker's yeast alpha-glucosidase (EC 3.2.1.20) and p-nitrophenyl-alpha-D-glucopyranoside (46 50). The samples (5 ug/mL) were prepared by dissolving the compounds 1-15 in DMSO. Test samples (10 uL) which had been prepared were reconstituted in 100 uL of phosphate buffer (100 mM) at pH 6.8 in 96-well microplate and incubated with 50 uL of baker's yeast alpha-glucosidase for 5 min before 50 uL of p-nitrophenyl-alpha-D-glucopyranoside (5 mM) was added. After incubating for 5 min, the absorbance was measured at 405 nm using SpectraMax plus384 (Molecular Devices Corporation, Sunnyvale, CA, USA). Blank in which the substrate was changed with 50 uL of buffer was analysed to accurately determine the background absorbance. Control sample was prepared to contain 10 uL DMSO instead of test samples.
Affinity data for this assay
 

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Last update November 1, 2007
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