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Assay Method Information

Assay Name:  Assay for α-Glucosidase Inhibitory Activity
Description:  The 135 ÁL of 50 mM phosphate saline buffer pH (6.8), solution of enzyme (20 ÁL), and 20 ÁL of test sample with 70% DMSO was added to 96-well plate. The samples were incubated for 15 min. Pre-read of the plate was taken after incubation using SpectraMax. substrate (pNPG) (25 ÁL) was added before final reading was takenat 400 nm on Spectra Max.
Affinity data for this assay
 

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Last update November 1, 2007
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