Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | LoxA Selectivity Assay |
| Description: | The one-point inhibition percentages were determined for human LOX inhibitors by following the formation of the conjugated diene product at 234 nm (ε = 25,000 M−1 cm−1) at an inhibitor concentration of 25 μM. All reaction mixtures were 2 mL in volume and constantly stirred using a magnetic stir bar at room temperature (22 °C) with 7 nM LoxA. Reactions were conducted in 25 mM HEPES buffer (pH 7.0), 0.01% Triton X-100, and 10 μM AA. The concentration of AA was quantitatively determined by allowing the enzymatic reaction to go to completion. Confirmed hitswere then screened against the human isozymes 5-LOX, 15-LOX-1, 15-LOX-2, and 12-LOX for specificity. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||