Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | Formaldehyde Dehydrogenase-Coupled Demethylase (FDH) Assay |
| Description: | For inhibition assays, powders of inhibitor compounds were dissolved in 100% (v/v) DMSO to give a 20 or 50 mM concentration and stored at -20 °C until needed. Various compounds with the concentrations ranging from 1.25 mM to 5 nM in a half-log serial dilution were pre-incubated with the reaction mixture for 15 min (0.5 mM enzyme [E], 15 mM peptide [S], 50 mM (NH4)2Fe(SO4)2, 1 mM αKG, and variable inhibitor [I] in the KDM5 reaction buffer with 10% DMSO). The addition of peptide and APAD+ initiated the reaction for 15 min at room temperature. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||