Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | GSK-3β activity assay |
| Description: | In a typical assay, 10 無 of test compound of different concentrations (dissolved in dimethyl sulfoxide [DMSO] and diluted with assay buffer) and 10 無 (20 ng) of enzyme were added to each well followed by 20 無 of assay buffer containing 25 lM substrate and 1 然 ATP. The final DMSO concentration in the reaction mixturewas less than 1%. After incubation at 30 蚓 for 30 min, the enzymatic reaction was quenched with 40 無 of Kinase-Glo reagent. Luminescence was recorded after 10 min using Infinite F200 PRO multimode reader (Tecan). |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||