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Assay Method Information |
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| | A1 Adenosine Receptor Binding Assay |
| Description: | Aliquots of cell membranes (30 ug proteins) were incubated at 25 °C for 180 min in 500 uL of binding buffer (50 mM Tris-HCl, 2 mM MgCl2, 2 units/mL ADA, pH 7.4) containing [3H]DPCPX (3 nM) and six different concentrations of the tested compounds. Non-specific binding was determined in the presence of 50 M R-PIA. The dissociation constant (Kd) of [3H]DPCPX in human A1 AR CHO cell membranes was 3 nM. |
| Affinity data for this assay | |
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