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| | In vitro AChE and BChE Assay |
| Description: | The synthesized compounds (each separately) were dissolved in a mixture of DMSO (1 ml) and methanol (9 ml) and then diluted in KH2PO4/K2HPO4 buffer (0.1 M, pH 8.0) to obtain desired concentration. The reaction mixture consisted of appropriate amount of phosphate buffer (pH 8.0), DTNB, test compounds, of 0.03 U/ml of enzymes (AChE and BChE). The mixture was pre-incubated at 30 °C for 10 min and followed by adding 1 mM ATCI or BTCI and incubated again for 15 min. The enzymatic hydrolysis was monitored at 412 nm using µQuant microplate spectrophotometer (MQX200, BioTek USA). Instead of inhibitor, DMSO was used asnegative control to subtract its effect on activity. All reactions were carried out in triplicate. The hydrolysis processes was monitored by Ellman's microplate method at 412 nm in the presence and absence of various concentrations of compounds at 30 °C. |
| Affinity data for this assay | |
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