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| | In vitro HDAC8 Assay |
| Description: | Briefly, the HDAC8 enzyme was diluted 20 times. Then, 10 μL of HDAC8 solution was mixed with compounds at various concentrations (50 μL). Then, 40 μL of the substrate [Boc-Lys-(acetyl)-AMC, 300 μm] was added, and the mixture was incubated at 37 °C for 30 min. Subsequently, the reaction was stopped by the addition of 100 μL of developer containing trypsin (10 μg/mL) and TSA (2 μm). Fluorescence intensity was measured by a microplate reader at excitation and emission wavelengths of 390 and 460 nm, respectively. |
| Affinity data for this assay | |
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