BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  In vitro HDAC8 Assay
Description:  Briefly, the HDAC8 enzyme was diluted 20 times. Then, 10 μL of HDAC8 solution was mixed with compounds at various concentrations (50 μL). Then, 40 μL of the substrate [Boc-Lys-(acetyl)-AMC, 300 μm] was added, and the mixture was incubated at 37 C for 30 min. Subsequently, the reaction was stopped by the addition of 100 μL of developer containing trypsin (10 μg/mL) and TSA (2 μm). Fluorescence intensity was measured by a microplate reader at excitation and emission wavelengths of 390 and 460 nm, respectively.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.