Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | Cholinesterase Inhibition Assay |
| Description: | Test compounds were prepared in DMSO (maximum concentration used 1% v/v), and 10 μL of each (0.001-25 μm final concentration range) was incubated for 5 min at room temperature with 160 μL of 1.5 mm DTNB, 50 μL 0.22 U/mL AChE [in 50 mm Tris-HCl, pH 8.0, 0.1% w/v bovine serum albumin (BSA)], or 50 μL 0.12 U/mL BuChE (in 50 mm Tris-HCl, pH 8.0, 0.1% w/v BSA). After the incubation period, 30 μLof ATChI (15 mm, prepared in ultrapure water) or BuTChI (15 mm, prepared in ultrapure water) was added to 96-well plates. Test compound inhibition of ChE was measured via UV absorbance (412 nm wavelength) using a microplate reader (SpectraMax M5) at various time intervals (t = 0, 1, 2, 3, 4, and 5 min). |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||