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| | Radioligand Binding Assay |
| Description: | Radioligand binding assays were performed using membranes prepared from CHO (hA1AR or hA3AR) or HEK293 (hA2AAR) cells stably expressing a single hAR subtype. The binding affinity for hA1, A2A and A3ARs was measured using a fixed (nM)concentration of an agonist radioligand, i.e. [3H]N6-R-phenylisopropyladenosine(~1.5), [3H]2-[p-(2-carboxyethyl)phenyl-ethylamino]-5′-N-ethylcarboxamidoadenosine(~16), or [125I]N-(4-amino-3- iodobenzyl)adenosine-5′-N-methyl-uronamide (~1.2), respectively. Nonspecific binding was determined using adenosine-′-Nethyluronamideat 10 μM concentration. |
| Affinity data for this assay | |
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