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Assay Method Information

Assay Name:  Substrate Binding Assay
Description:  The interaction of 3HB6H (25 uM) with substrate analogs was studied in 50 mM Tris-SO4 (pH 8.0). Dissociation constants (Kd) of enzyme-substrate complexes were determined from flavin absorption difference spectra essentially as described elsewhere [Montersino et al., Biochim. Biophys. Acta, 1824:433-442].
Affinity data for this assay
 

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Last update November 1, 2007
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