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Assay Method Information |
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| | Equilibrium Dialysis |
| Description: | Equilibrium dialysis was performed using purified recombinant CtBP proteins (10 μM) in binding buffer (25 mM Tris-Cl, 100 mM NaCl, 1 mM EDTA, 5% glycerol, 1 mM DTT) on one side of a 10-kDa molecular mass cutoff membrane with either [adenine-2,3-3H]NAD+ or [4-3H]NADH in varying concentrations from 12 nM to 50 μM at 4 °C for 48 h. |
| Affinity data for this assay | |
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