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Assay Method Information

Assay Name:  Equilibrium Dialysis
Description:  Equilibrium dialysis was performed using purified recombinant CtBP proteins (10 μM) in binding buffer (25 mM Tris-Cl, 100 mM NaCl, 1 mM EDTA, 5% glycerol, 1 mM DTT) on one side of a 10-kDa molecular mass cutoff membrane with either [adenine-2,3-3H]NAD+ or [4-3H]NADH in varying concentrations from 12 nM to 50 μM at 4 C for 48 h.
Affinity data for this assay
 

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Last update November 1, 2007
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