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Assay Method Information |
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| | In Vitro HotSpot Kinase Assay |
| Description: | IC50s were determined using the in vitro HotSpot kinase assay (purified enzymes, 33P-ATP, an appropriate substrate and 1 uM ATP.). Reaction conditions were 1 uM ATP, one hour incubation with inhibitor, and kinase activity detected using 33-ATP phosphorylation of an appropriately selected peptide substrate. |
| Affinity data for this assay | |
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