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| | Baker's Yeast α-Glucosidase Inhibition Assay |
| Description: | The enzyme inhibition was evaluated according to the method previously reported by Zawawi et al. [Zawawi et al., Bioorg. Chem., 23:3119] with slight modification. Various concentration of test compounds (10 μL) were dissolved in DMSO (ranging from 200 to 6.25 μg/mL) and premixed with 95 μL of 50 mM phosphate buffer (pH 6.8). Then, 25 μL of enzyme (0.0625 U/mL) in phosphate buffer saline was added into each well and the plate was incubated at 37 °C for 10 min. Afterward, 25 μl of PNPG in phosphate buffer saline (5 mM) were added and pre-read of the plate was taken by using a microplate reader (Spectrostar Nano BMG Labtech, Germany). The reaction mixture was then incubated at 37 °C for 30 min and change in absorbance at 405 nm was monitored up to 30 min. For negative control, the test samples were replaced with 10 μL of DMSO and acarbose was used as positive control. All experiments were triplicated and the results were expressed as the mean ± S.E.M of three determinations. |
| Affinity data for this assay | |
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