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Assay Method Information

Assay Name:  α-Glucosidase Inhibition Assay
Description:  At 37 C, after incubation of 10 min of a reaction mixture solution of 70 μL (50 mM) phosphate buffer of 6.8 pH, 10 μL (0.5 mM) of a test compound and 10 μL (0.0234 units, Sigma Inc.) of enzyme, the absorbance was pre-read at 400 nm. Afterwards, 10 μL of 0.5 mM p-nitrophenyl-a-d-glucopyranoside (code No. N1377 fromSigma Inc) was added to instigate the reaction. Later 30 min incubation at 37 C, yellow coloured p-nitrophenol was formed and the absorbance was again recorded at 400 nm to see the difference. These tests were performed in triplicates and suitable dilutions of samples were made for screening.
Affinity data for this assay
 

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Last update November 1, 2007
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