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Assay Method Information

Assay Name:  CK2α Inhibition Assay
Description:  The activity of CK2α was tested using P81 filter isotopic assay, as it was described earlier [Łukowska-Chojnacka et al., Bioorg. Med. Chem., 24:735-741]. IC50 values for studied compounds were determined with minimum 7 concentrations of each tested inhibitor ranging from 0.064 to 1000 mM (in the presence of 4% DMSO) and calculated by fitting the data to sigmoidal doseresponse (variable slope) Y = Bottom + (Top-Bottom)/(1 + 10^((LogIC50-X) * HillSlope) equation in GraphPad Prism. Ki values were calculated using Cheng and Prusoff [29] equation: Ki = IC50/(1 + [S]/Km), [S] = 10 mM ATP, Km = 11 mM.
Affinity data for this assay
 

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Last update November 1, 2007
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